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Researchers Find Color-Sensitive
Atomic Switch in Bacteria
Discovery sheds light on cell membranes, the most accessible targets for medications
Researchers using extremely high resolution imaging have found an atomic switch capable of discriminating color in a bacterial membrane protein.
John L. Spudich, Ph.D.
In a paper posted Sept. 30 on Science Express, the rapid advance publication page of Science, scientists from The University of Texas Medical School at Houston and the University of California, Irvine, described the versatile light-sensing protein at levels of resolution smaller than a nanometer - one billionth of a meter.
"High-resolution X-ray crystallography revealed the light-absorbing part of the protein was present in two alternative positions, suggesting to us that light of different colors drives this protein back and forth between two differently colored states of the protein," said co-senior author John L. Spudich, Ph.D., director of the Center for Membrane Biology in the UT Medical School Department of Biochemistry and Molecular Biology.
"Chemical analysis and spectroscopic methods then proved that the switch, buried in the middle of this membrane-embedded protein, similar in structure to our visual pigments, is controlled by blue versus orange photon absorption," Spudich said.
That function makes the protein unusual among its family of light-sensing proteins known as rhodopsins, which are present in microbes and higher animals. In human eyes, rhodopsin is the light-absorbing pigment of the rods, located in the retina. The team studied a new-found rhodopsin on the surface membrane of the bacterium Anabaena, classified as "blue-green algae" or cyanobacteria, which rely on photosynthesis to generate energy.
Having a single sensory protein capable of distinguishing color would provide Anabaena with information about the color of light available in its environment, enabling more efficient harvesting of light for photosynthesis, Spudich said.
"Understanding rhodopsins helps us understand the large number of related membrane receptors involved in cell signaling that govern biological functions," Spudich said. In the longer term, the novel protein found in Anabaena has the potential to be used in nanomachinery as a color-sensor; however, the authors point out that this practical application is years in the future.
"One of the key frontiers of biomedical science in the genomic era is the crucial role of cell membranes in normal cell function and disease states," said Spudich, who holds the Robert A. Welch Distinguished Chair in Chemistry and is a professor in the UT Graduate School of Biomedical Sciences at Houston. "Ask virtually any investigator, and you'll find his or her research program bumps up against a membrane."
Cell membrane surfaces and their exposed proteins are the most accessible targets to treat human tissue or destroy infectious microbes, he said. More than 60 percent of medications target membrane proteins on human cells, and many antibiotics target membranes on pathogens.
First author of the paper is Lutz Vogeley, a graduate student in the UC Irvine Department of Molecular Biology and Biochemistry. Senior authors are Spudich and Hartmut Luecke, Ph.D., professor of molecular biology and biochemistry and of physiology and biophysics at UC Irvine. Co-authors include Oleg Sineshchekov, Ph.D., of Moscow State University in Russia and visiting professor in the UT Center for Membrane Biology; and research fellow Vishwa Trivedi, Ph.D., and assistant professor Jun Sasaki, Ph.D., both of the UT Center for Membrane Biology.
By Scott Merville, Public Affairs